12/3/2023 0 Comments Adrenaline energy drink![]() Finally, concentration addition modeling using quantitative data from the 6 common ingredients and multivariate prediction modeling was used to determine potential ingredients responsible for the adverse effects on the cardiomyocytes. We found that some of the energy drinks elicited adverse effects on the cardiomyocytes with the most common being an increase in the beat rate, while QT prolongation was also observed at the lowest concentrations. Data on the beat rate (positive and negative chronotropy), ion channel function (QT prolongation), and cytotoxicity were collected in a dilution series. In vitro effects of energy drinks and some of their ingredients were then tested in iPSC-derived cardiomyocytes. Approximately 300 features were observed across samples in the untargeted studies, and of these ~100 were identified. In addition, untargeted analyses for each beverage were performed with a platform combining LC, ion mobility spectrometry and mass spectrometry (LC-IMS-MS) measurements. The concentrations of six common ingredients (caffeine, taurine, riboflavin, pantothenic acid, adenine, and L-methionine) were quantified by coupling liquid chromatography with a triple quadrupole mass spectrometer for the acquisition of LC-MS/MS spectra. Energy drinks from 17 widely-available over-the-counter brands were evaluated in this study. ![]() ![]() We therefore characterized the chemical profiles and in vitro effects of energy drinks and their ingredients on human induced pluripotent stem cell (iPSC)-derived cardiomyocytes, and identified the putative active ingredients using a multivariate prediction model. Consumption of energy drinks has been associated with adverse cardiovascular effects however, little is known about the ingredients that may contribute to these effects.
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